Plasma Lipids and Platelet Membrane Fluidity in Essential Hypertension

Kim Hanh Le Quan Sang; Madeleine Mazeaud; Catherine Astarie; Véronique Duranthon; Fathi Driss; Marie-Aude Devynck

doi:10.1055/s-0038-1651551

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1993; 69(01): 070-076
DOI: 10.1055/s-0038-1651551

Original Article

platelets

Schattauer GmbH Stuttgart

Plasma Lipids and Platelet Membrane Fluidity in Essential Hypertension

Kim Hanh Le Quan Sang

¹Pharmacology, CNRS URA 1482, Necker Medical School, Paris, France

,

Madeleine Mazeaud

¹Pharmacology, CNRS URA 1482, Necker Medical School, Paris, France

,

Catherine Astarie

¹Pharmacology, CNRS URA 1482, Necker Medical School, Paris, France

,

Véronique Duranthon

²Service de Biochimie, Hôpital Saint-Antoine, Paris, France

,

Fathi Driss

²Service de Biochimie, Hôpital Saint-Antoine, Paris, France

,

Marie-Aude Devynck

¹Pharmacology, CNRS URA 1482, Necker Medical School, Paris, France

› Author Affiliations

Abstract

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Summary

Essential hypertension is often associated with high levels of plasma cholesterol or triglycerides. The relationships between plasma lipids and platelet lipids, membrane fluidity and functions in untreated hypertensive patients were investigated by measuring the fluorescence anisotropies of two fluorescent dyes (DPH and its cationic derivative, TMA-DPH, with different subcellular localization), cytosolic Ca²⁺ and pH, cyclic AMP content and aggregation to ADP and collagen. Hypercholesterolemia was found to be accompanied by a rise in platelet cholesterol content without changes in TMA-DPH or DPH anisotropies whereas hypertriglyceridemia was associated with a decreased cholesterol to phospholipid molar ratio, a decreased DPH anisotropy and a tendency of the cytosol to alkalinize. These results point out the differences between the effects of an acute cholesterol load and those of chronic hypercholesterolemia on platelet membrane microviscosity and aggregation. They demonstrate a strong association between plasma triglyceride levels and platelet membrane structure.

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References

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